Human Mdm2 p53 Binding Protein Homolog (MDM2) ELISA Kit

Researchers investigating the p53 tumor suppressor pathway rely on accurate quantification of MDM2, the principal E3 ubiquitin-protein ligase that negatively regulates p53 stability. This ELISA Kit from Assay Genie is validated for research use only and supports measurement of human Mdm2 p53 Binding Protein Homolog in tissue homogenates and cell lysates.

Validated workflows span oncology research, apoptosis studies, and DNA damage response investigations, particularly where researchers examine MDM2 amplification in tumor samples, evaluate compound effects on the p53-MDM2 interaction, or monitor MDM2 upregulation following genotoxic stress. Sample types include tissue homogenates prepared from cultured cells or xenograft material, as well as cell lysates from treated and untreated cell populations.

The kit supplies reagents for 96 assays in a microplate format. The sealed microplate and standard diluent should be refrigerated upon arrival. Stock components intended for frozen storage at -20°C should remain frozen until first use, with freeze-thaw cycles limited to preserve reagent activity. The streptavidin-HRP conjugate should be protected from light and stored refrigerated.

Applications include confirming pathway modulation alongside Western blot analysis, comparing baseline MDM2 levels across tumor and matched normal tissue homogenates, and supporting in vitro cell culture experiments. The assay format also complements qPCR and immunoprecipitation studies of p53 pathway activity, enabling cross-validation of MDM2 expression changes under different experimental conditions.