Human MDH1 ELISA Kit (HUFI07654)

Cytoplasmic malate dehydrogenase (MDH1) catalyzes the reversible NAD+/NADH-dependent interconversion of malate and oxaloacetate, supporting the citric acid cycle, gluconeogenesis, and cellular redox balance. Researchers studying cancer metabolism, mitochondrial biology, and metabolic disease pathways require sensitive quantification of MDH1 protein levels in human specimens.

The Assay Genie ELISA Kit provides a microplate-based immunoassay for measuring MDH1 in human serum samples or EDTA-plasma specimens. Each kit contains pre-coated wells, recombinant MDH1 calibrators, enzyme-conjugated detection antibodies, wash buffer concentrate, substrate, and stop solution for complete workflow setup. Reagents are supplied in individual vials for convenient handling.

The protocol uses 100 uL sample volumes per well for absorbance-based quantification, with standard curves generated from recombinant MDH1 calibrators enabling interpolation of unknown concentrations. Each kit is configured for 96 determinations, supporting medium-throughput studies.

Intended for in vitro research use, this kit supports biomarker discovery, pathway analysis, and disease mechanism studies. Store reagents refrigerated between uses to preserve activity and reproducibility. All components are configured for research applications and should not be used for diagnostic procedures.