Human MDC1 ELISA Kit (HUFI07660)

MDC1 (Mediator of DNA Damage Checkpoint 1), also called NFBD1, is a nuclear phosphoprotein that serves as a scaffold in the cellular DNA damage response, recruiting repair factors such as 53BP1, BRCA1, and the MRE11-RAD50-NBS1 complex to double-strand break sites. Quantitative measurement of MDC1 protein levels in human specimens supports research into genomic stability, checkpoint signaling, tumor biology, and cellular responses to ionizing radiation.

This ELISA Kit from Assay Genie is configured for 96 Assays and provides an in vitro tool for laboratories investigating DNA damage and repair pathways. The procedure yields a measurable colorimetric signal proportional to MDC1 concentration in the prepared specimen, and the workflow accommodates samples derived from serum or plasma for translational research applications.

All kit components should be stored refrigerated upon arrival, and reagents remain stable through the expiration date printed on the label when handled according to the supplied protocol. The kit is intended for research use only and is not validated for diagnostic or clinical applications. Researchers are advised to avoid freeze-thaw cycles of prepared samples and to allow all reagents to equilibrate to room temperature before beginning the assay workflow.