Human MCP3/CCL7 ELISA Kit

A high-quality Sandwich ELISA kit from Assay Genie designed for the precise quantification of MCP-3 (also known as CCL7) in human samples. MCP-3 is a chemokine that plays a vital role in immune response regulation and inflammation, recruiting leukocytes to sites of infection or injury. It is therefore a valuable marker for research into inflammatory conditions, autoimmune diseases, and cancer progression.

The assay uses a double-antibody sandwich format with colorimetric detection, offering a sensitivity of 9.375 pg/mL and a working range of 15.625–1000 pg/mL. It is validated for use with human serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. Reagents should be stored at 2–8°C, where they remain stable for up to 12 months.

The protocol involves sequential incubations with a biotin-labelled detection antibody and an HRP-Streptavidin conjugate, followed by TMB substrate development and absorbance reading at 450 nm. Linearity has been demonstrated across serum, EDTA plasma, and heparin plasma samples, with strong recovery performance reported in all three matrices.