Human MCP/CD46 (Membrane Cofactor Protein) ELISA Kit (HUES02730)

From Assay Genie, the workflow uses biotinylated detection antibody and streptavidin-HRP with TMB substrate, producing measurable absorbance at 450 nm on a standard plate reader after the reaction is stopped with acid. The standard curve spans roughly 0.16 to 10 ng/mL, allowing interpolation of unknown sample concentrations, and intra-assay precision is typically within 10% CV. Serum and plasma may be diluted directly, while conditioned media should be clarified at 1000 x g for 10 minutes prior to loading.

Total assay time is approximately 3.5 hours at room temperature. Sealed kits should be stored at 2-8 °C until first use; reconstituted standards and concentrated conjugate are best held at -20 °C, and repeated freeze-thaw of prepared reagents should be avoided. The 96-well microplate, standard vials, detection conjugate, substrate, and wash buffer together support a complete single-use layout for MCP/CD46 quantification in research use only applications spanning complement regulation, atypical hemolytic uremic syndrome, transplantation immunology, and viral receptor studies.