Human MCOLN1 (Mucolipin-1) ELISA KIT (HUFI08127)

The Human MCOLN1 (Mucolipin-1) ELISA Kit from Assay Genie supports quantitative detection of mucolipin-1, a TRPML subfamily ion channel that resides on late endosomal and lysosomal membranes. Loss-of-function mutations in the MCOLN1 gene cause mucolipidosis type IV, a neurodegenerative lysosomal storage disorder, making this kit useful for studies of endolysosomal trafficking, autophagy regulation, and intracellular calcium release. Researchers can pair the assay with siRNA knockdown experiments, CRISPR-edited cell models, or TRPML channel agonist dose-response curves to explore mucolipin-1 turnover in patient-derived fibroblasts and other relevant cell lines. Each kit contains pre-coated microplate wells, biotinylated detection reagent, streptavidin-HRP conjugate, calibrated standard diluent, and substrate solution for the complete workflow. Reagents are shipped on cold packs and should be refrigerated upon arrival; avoid repeated freeze-thaw cycles of the detection antibody to preserve activity. The assay is intended for research use only and is not validated for diagnostic procedures.