Human MCM5 (DNA replication licensing factor MCM5) ELISA Kit (HUFI07666)

MCM5 is a minichromosome maintenance protein that functions as a DNA replication licensing factor, part of the MCM2-7 helicase complex that prepares chromatin for S-phase entry. This quantitative immunoassay from Assay Genie is built for researchers measuring endogenous MCM5 concentrations in human clinical specimens using a microplate format with absorbance-based readout at the end of the run.

The kit targets the DNA replication licensing factor MCM5, which has been investigated as a urinary biomarker for bladder cancer screening and as a proliferation marker in dysplastic tissue. Scientists working on cell cycle regulation, replicative stress, oncology biomarker development, and genitourinary disease models use this assay to quantify circulating MCM5 levels alongside complementary proliferation markers such as Ki-67 and PCNA.

Each kit contains a pre-coated plate, biotinylated detection antibody, recombinant standards, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent sufficient for 96 determinations. The assay follows a standard curve-based protocol, with results interpolated from a recombinant MCM5 standard curve and reported in ng/mL after correction for any sample dilution.

For laboratory research use only, the kit components should be stored refrigerated and equilibrated to room temperature before use. Researchers are advised to avoid repeated freeze-thaw cycles of the reconstituted standards and to aliquot working reagents when working across multiple days. Reagent volumes are sized for full-plate runs, and partial-plate users should plan accordingly.