Human MCL1 ELISA Kit

From Assay Genie, this Human MCL1 ELISA Kit is a colorimetric Sandwich ELISA, Double Antibody assay designed for the precise quantitative measurement of MCL1 in human samples. The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is intended for research in the Cell Death area. MCL1 is described as a key protein that plays a critical role in regulating apoptosis and cell survival and has been implicated in diseases such as cancer and autoimmune disorders.

The assay has a stated sensitivity of 18.75 pg/mL with a detection range of 31.25-2000 pg/mL, and kit components should be stored at 2-8°C for 12 months. Reported linearity for serum (n=5) is 87-102% at a 1:2 dilution, 86-97% at 1:4, and 81-96% at 1:8; EDTA plasma shows 87-104%, 82-100%, and 85-96% at the same dilutions, while heparin plasma shows 85-101%, 84-101%, and 83-100%. Recovery averages 94% for serum, 92% for EDTA plasma, and 93% for heparin plasma across the tested sample set.

The procedure involves reagent and plate preparation, a primary incubation at 37°C for 90 minutes, detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, color development with TMB substrate in the dark for 10–20 minutes, and a stop reaction with absorbance measurement at 450 nm.