Human MCAM/CD146 PharmaGenie ELISA Kit (SBRS0860)

MCAM/CD146 is a cell-surface immunoglobulin superfamily glycoprotein expressed on vascular endothelium, mesenchymal stem cells, and multiple tumor cell populations, where it functions as a biomarker for angiogenesis, tumor invasion, and metastatic progression. Quantification of this antigen in human specimens supports research in vascular biology, oncology, and regenerative medicine, including melanoma and breast carcinoma studies.

The Assay Genie Human MCAM/CD146 PharmaGenie ELISA Kit is a research-use-only in vitro immunoassay tool configured for 96 determinations per kit. It is intended for quantitative measurement of MCAM/CD146 in cell culture supernatant samples and additional human-derived specimen matrices. The procedure involves applying diluted standards and unknown samples to the pre-coated plate, completing the assay workflow, and reading absorbance values on a standard microplate reader to interpolate antigen concentrations from a generated standard curve.

Each kit is supplied with the essential reagents and a protocol insert. Store kit components refrigerated and protect from light to preserve reagent activity, equilibrate reagents to room temperature before use, and minimize freeze-thaw cycles of reconstituted standards to maintain assay performance.