Human MC5R (Melanocortin 5 Receptor) ELISA Kit (HUES03482)

The Melanocortin 5 Receptor (MC5R) is a G-protein-coupled receptor expressed in exocrine tissues, sebaceous glands, and adipose, where it mediates melanocortin peptide signaling related to thermoregulation and energy balance. This Assay Genie sandwich ELISA enables quantitative measurement of MC5R in serum, plasma, and other biological fluids with a colorimetric readout at 450 nm on a 96-well microplate.

Each kit includes a pre-coated plate, biotinylated detection antibody, streptavidin-HRP conjugate, and a recombinant human MC5R standard with an analytical sensitivity in the low pg/mL range. The protocol shows negligible cross-reactivity with related melanocortin receptors and is validated for intra- and inter-assay precision. Unopened components should be stored at 2-8 °C; reconstituted standards and working solutions can be aliquoted and frozen to minimize freeze-thaw cycles. Intended for research use only, this ELISA supports investigations of dermatological biology, metabolic disorders, and GPCR signaling pathways.