Human MBP (Myelin Basic Protein) CLIA Kit (HUES00141)

Designed by Assay Genie for the precise and reliable measurement of Myelin Basic Protein (MBP) in human serum, plasma, and cell culture supernatants, this chemiluminescence immunoassay kit supports research into neurological disorders such as multiple sclerosis and other demyelinating diseases. MBP is a key component of the myelin sheath, essential for proper functioning of the nervous system, and abnormal levels are associated with demyelinating conditions.

The kit operates on a sandwich chemiluminescence immunoassay (CLIA) principle, using a microplate pre-coated with an antibody specific to the target protein. Detection is by chemiluminescence, with a biotinylated detection antibody and HRP-conjugated streptavidin forming a sandwich complex, and signal intensity measured using a luminometer. The reported assay time is 3 h 30 min, with a sensitivity of 4.69 pg/mL and a detection range of 7.81–500 pg/mL. Recovery is stated as 80%–120%.

Intended for use with human serum, plasma and other biological fluids at a sample volume of 100 μL, the assay recognises Human MBP with no significant cross-reactivity or interference from analogues reported. The product falls within the research areas of Cancer, Neuroscience, and Tags and Cell Markers, and components should be stored at 2–8 °C or –20 °C as indicated.