Human MBLAC1/Metallo-beta-lactamase domain-containing protein 1 ELISA Kit

A high-quality sandwich ELISA, double antibody immunoassay from Assay Genie designed for the quantitative measurement of human MBLAC1 (Metallo-beta-lactamase domain-containing protein 1) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a pre-coated 96-well microplate to capture the target, a biotinylated detection antibody, and HRP-conjugated Streptavidin with TMB substrate for colorimetric readout at 450 nm. A Bradford Reagent is included to support total protein quantification and sample normalization. The kit is shipped on ice packs and components are stored at 2-8°C, with stability up to 12 months.

Key features reported by Assay Genie include a sensitivity of 46.875 pg/mL and a working range of 78.125-5000 pg/mL. Linearity has been evaluated in serum (90-100% at 1:2, 82-99% at 1:4, 84-95% at 1:8), EDTA plasma (84-98% / 90-98% / 92-99%), and heparin plasma (86-99% / 85-101% / 86-99%). Spike-and-recovery across the same matrices ranges from 90-105% (avg 94%) in serum, 86-99% (avg 94%) in EDTA plasma, and 89-96% (avg 96%) in heparin plasma. The assay procedure involves equilibrating reagents, a 90-minute primary incubation at 37°C, a 60-minute biotinylated detection antibody incubation, a 30-minute HRP-Streptavidin incubation, TMB development in the dark for 10-20 minutes, and absorbance reading at 450 nm after stop solution. The kit is intended for research use only.