Human MBD4 ELISA Kit (HUFI03488)

Methyl-CpG binding domain protein 4 (MBD4) is a thymine and uracil DNA glycosylase that operates within the base excision repair pathway, correcting G:T and G:U mispairs generated by spontaneous deamination of 5-methylcytosine at CpG dinucleotides. The Assay Genie human MBD4 ELISA Kit enables quantitative measurement of this protein in human serum or plasma specimens, supporting research into epigenomic maintenance, DNA repair capacity, and the molecular biology of mismatch repair-deficient tumors. Loss-of-function mutations in MBD4 have been reported in colorectal and endometrial cancers with deficient mismatch repair, where the absence of functional MBD4 contributes to elevated somatic mutation rates and the microsatellite instability phenotype observed in Lynch syndrome.

Configured for 96 Assays per kit, this immunoassay delivers colorimetric readouts on a standard microplate reader and supplies all components required to construct a calibration curve and process study samples. Pre-coated microwell strips, calibrated reference standards, detection antibody, substrate, and wash buffer are packaged together in a single kit, allowing laboratories to integrate MBD4 quantification into existing biomarker workflows. Investigators characterizing mutational signatures, methylation-driven genome instability, or patient stratification in gastrointestinal and endometrial oncology can apply the kit to compare circulating MBD4 levels between healthy donors and affected cohorts in translational research programs.