Human MBD2 ELISA Kit (HUFI03486)

Quantify Methyl-CpG-binding domain protein 2 (MBD2) in human research samples with this ELISA kit from Assay Genie. MBD2 is a chromatin-associated reader of methylated CpG dinucleotides and a key participant in DNA methylation-dependent transcriptional repression, making it a focal target in studies of epigenetic regulation, X-inactivation, and tumor suppressor gene silencing.

The assay is configured for in vitro measurement of human MBD2, supporting investigations in cancer biology, developmental biology, and chromatin biology. MBD2 expression has been characterized in colorectal carcinoma, breast carcinoma, and acute myeloid leukemia contexts, where altered methylation patterns and aberrant reader protein levels contribute to disease phenotypes. Researchers studying methyltransferase activity, methyl-CpG binding dynamics, or NuRD complex function will find MBD2 quantification useful for tracking chromatin regulatory changes across experimental conditions.

Reagents ship on cold packs and should be stored refrigerated upon arrival to preserve antibody and standard activity. Allow all components to equilibrate to room temperature before use, and avoid repeated freeze-thaw cycles of the reconstituted standard. Unopened kit components remain stable through the printed expiration date when handled as directed on the vial labels.

Assay Genie manufactures this research use only kit under standard QC protocols and supplies a microplate pre-coated with capture antibody, biotinylated detection reagent, streptavidin-HRP conjugate, recombinant MBD2 standards, and assay diluent. Consult the supplied manual for full procedural details, dilution recommendations, and expected performance characteristics for your specific sample matrix.