Human Maternal embryonic leucine zipper kinase (MELK) ELISA Kit

From Assay Genie, this sandwich ELISA Kit is designed for the quantitative measurement of Maternal Embryonic Leucine Zipper Kinase (MELK) in human serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. It detects natural and recombinant human MELK and is for research use only, with applications in cancer research.

The assay has a reported detection range of 0.156-10 ng/mL with a sensitivity of 0.098 ng/mL. Reported precision figures include intra-assay CV of 4.9% and inter-assay CV of 8.4%. Linearity was evaluated in serum, EDTA plasma and heparin plasma across dilutions of 1:2, 1:4, 1:8 and 1:16. Recovery was 86% (range 80-92%) in serum and 88% (range 82-94%) in plasma.

MELK is a serine/threonine-protein kinase involved in cell cycle regulation, stem cell self-renewal, apoptosis and splicing regulation. The protein functions as a monomer and interacts with ZNF622 and PPP1R8. Recognised synonyms include Protein kinase Eg3, Protein kinase PK38, Tyrosine-protein kinase MELK, pEg3 kinase, hPK38, hMELK and KIAA0175.