Human MAPK1 (Mitogen-activated protein kinase 1) ELISA Kit (HUFI07674)

Quantitative detection of MAPK1 (ERK2), a key serine/threonine kinase in the RAS-RAF-MEK-ERK signal transduction cascade, is enabled by the Assay Genie Human MAPK1 ELISA Kit. This enzyme transmits signals from growth factors, cytokines, and stress stimuli into proliferation, differentiation, and survival responses across many tissue types.

Investigators studying oncogenic signaling use this kit to explore pathway dysregulation in melanoma, colorectal carcinoma, and thyroid cancers where constitutive MAPK activation drives tumor progression and acquired resistance to targeted therapies. The assay additionally supports inflammatory disease research, neurobiology studies of synaptic plasticity, and pharmacological screening of kinase inhibitors in translational programs.

Each kit provides pre-coated plate strips, calibrator standards, detection reagents, substrate solution, and wash buffer sufficient for the stated number of determinations. Signal quantification requires a standard microplate reader capable of absorbance measurement. Reconstituted components and working dilutions should be kept refrigerated during use, and unused kit components stored cold to preserve activity across the stated shelf life.

Applications include pathway activation profiling across patient cohorts, longitudinal monitoring of treatment response in clinical research, and validation of MAPK1 as a candidate biomarker in proliferative and inflammatory disease studies.