Human MAP4K2 ELISA Kit (HUFI03514)

Quantitative measurement of MAP4K2, a serine/threonine kinase within the STE20 family, supports research into stress-activated MAPK signaling cascades, apoptosis regulation, and lymphocyte activation mechanisms. The kit is configured as a complete assay pack containing all reagents required for a single microplate run, delivering a colorimetric read-out compatible with standard absorbance plate readers. Investigators examining JNK pathway cross-talk, germinal center B-cell biology, or inflammatory signaling networks can apply this kit to human donor specimens collected in serum tubes or plasma tubes.

Assay Genie supplies each kit with a pre-coated microplate, detection antibody vial, standard diluent, wash buffer concentrate, substrate solution, and stop reagent. Components arrive ready for use following brief dilution of the wash buffer and reconstitution of the lyophilized standards. The protocol follows an incubation-based workflow with sample binding, detection antibody binding, substrate development, and absorbance measurement, enabling researchers to generate a standard curve and interpolate unknown sample concentrations against the included reference preparation.

This MAP4K2 ELISA Kit is intended for research use only and supports investigations of T-cell receptor signaling, oxidative stress responses, and tumor necrosis factor pathway biology. Each kit provides one microplate, allowing processing of up to 96 determinations in duplicate, accommodating both small pilot studies and larger screening campaigns.