Human MAP2K2 (Dual specificity mitogen-activated protein kinase kinase 2) ELISA KIT (HUFI08125)

The MAP2K2 protein, also referred to as MEK2, is a ~44 kDa dual-specificity kinase that phosphorylates ERK1 and ERK2 within the RAS-RAF-MEK-ERK signal transduction cascade. Aberrant MAP2K2 activity is investigated in melanoma, colorectal carcinoma, and cardio-facio-cutaneous syndrome research, where it serves as both a therapeutic target and a biomarker of pathway activation.

This Assay Genie colorimetric immunoassay arrives in a microplate format intended for research use only, with a standard curve covering 0.156–10 ng/mL and requiring 100 µL of sample per well. Reconstituted standards and biotinylated detection antibody should be aliquoted and frozen for repeated use; unopened components are stored refrigerated and protected from light. Allow all reagents to reach room temperature before initiating the protocol to ensure consistent capture-antibody binding across the well plate.