Human MANSC Domain Containing Protein 1 (MANSC1) ELISA Kit

This quantitative immunoassay from Assay Genie enables researchers to profile human MANSC1 levels in cultured samples and tissue-derived specimens for in vitro work. The MANSC domain is a small conserved motif identified in a limited set of transmembrane and secreted proteins, and quantitative detection of MANSC1 supports investigations into its tissue-specific abundance and its potential roles in developmental regulation. Each kit is configured for 96 determinations and is supplied with pre-coated plate strips, lyophilized recombinant standards, a biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, TMB substrate, and stop solution. Briefly, standards and samples are added to the coated wells; after incubation and washing, the detection antibody binds the captured analyte and the HRP-TMB reaction is developed, then the absorbance is read on a standard microplate reader. Reconstituted standards should be stored refrigerated and aliquoted to limit repeated freeze-thaw cycles, while the conjugate must be protected from light during use. The protocol supports multiple human specimen types, including clinical serum samples, plasma-derived materials, tissue homogenates, and clarified cell lysates from cell culture, giving laboratories flexibility across diverse study designs. Intended for research use only.