Human Mannosidase Alpha Class 1A Member 1 (MAN1A1) ELISA Kit

Quantitative immunoassay for measuring Mannosidase Alpha Class 1A Member 1 (MAN1A1) in human research samples. MAN1A1 is a Golgi-resident type II transmembrane enzyme that initiates trimming of N-linked glycans by hydrolyzing the terminal alpha-1,2-mannose residue from Man9GlcNAc2 to generate Man8GlcNAc2 isomers, a step upstream of calnexin and calreticulin quality control and ER-associated degradation of misfolded glycoproteins. The kit from Assay Genie is configured for 96 individual reactions and is intended for use with tissue homogenates and similar biological preparations. Researchers studying congenital disorders of glycosylation, hepatocellular endoplasmic reticulum stress, and glycoprotein processing pathways use this assay to track enzyme abundance across cultured cell lines and primary tissue. Workflow involves adding prepared samples and standards to the pre-coated plate, followed by a biotinylated detection antibody, streptavidin-HRP conjugate, and a chromogenic substrate; absorbance is then read on a standard microplate reader. Each kit ships with a pre-coated plate, detection reagents, calibrated standards, and assay buffer sufficient to complete the full plate. The product is intended for research use only and is not validated for diagnostic procedures.