Human MANEA ELISA Kit (HUFI03519)

Encoded by the MANEA gene, Mannosidase Endo-Alpha is a Golgi-resident hydrolytic enzyme that trims N-linked glycoproteins and has emerged in published studies as a contributor to hepatic lipid handling and metabolic disease phenotypes. The Assay Genie kit is a quantitative, two-antibody immunoassay designed for research use only, supplied as a complete microplate-format package containing a pre-coated well plate, recombinant MANEA calibration standards, biotinylated detection antibody, streptavidin-enzyme conjugate, wash concentrate, chromogenic substrate, and stop solution. Reagents are sufficient for 96 individual determinations, and the workflow follows an add-sample-incubate-wash-develop-read sequence with absorbance readout. The kit is intended for quantifying endogenous human MANEA in cell lysates, tissue homogenates, and clarified serum or plasma aliquots, supporting research applications in glycoprotein processing, biomarker development, and metabolic pathway characterization across academic, pharmaceutical, and translational laboratories.