Human MAMLD1 (Mastermind-like domain-containing protein 1) ELISA KIT (HUFI05075)

The Mastermind-like domain-containing protein 1 (MAMLD1) is an X-linked transcriptional coactivator implicated in urogenital development, with loss-of-function mutations linked to hypospadias and 46,XY disorders of sex development. This quantitative immunoassay from Assay Genie enables researchers to measure MAMLD1 protein levels in human circulating specimens, supporting investigations in reproductive endocrinology, pediatric urology, and sex-differentiation research.

The kit includes a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent sufficient for the stated assay count. Researchers will need to supply a plate reader capable of absorbance measurement. The assay follows a standard two-antibody capture workflow and is intended for research use only, in vitro applications.

MAMLD1 is expressed in testis, ovary, and other steroidogenic tissues, and altered expression has been reported in studies of androgen biosynthesis and gonadal function. Investigators studying the molecular basis of hypospadias, gender assignment outcomes, or androgen receptor signaling pathways may use this kit to quantify MAMLD1 levels in patient cohorts and matched controls. Detailed standard curve information, sample dilution recommendations, and intra-/inter-assay precision data are provided in the enclosed protocol insert.