Human MAML1 (Mastermind-like protein 1) ELISA Kit (HUFI03513)

The MAML1 protein functions as a key transcriptional coactivator within the Notch signaling cascade, binding the intracellular domain of Notch receptors (NICD) and the CSL/RBPJ transcription factor to drive expression of downstream target genes such as HES1 and HEY1. Researchers investigating hematopoiesis, T-cell lineage commitment, lymphocyte maturation, or Notch-driven oncogenesis in breast, lung, and colorectal cancers rely on quantitative MAML1 readouts from cell lysates and clinical specimens. This Assay Genie microplate kit provides a reproducible ELISA workflow, with all buffers, antibodies, and standards supplied for 96 individual determinations. Cell lysate preparations, tissue homogenates, and patient-derived serum or plasma can be processed using the capture-antibody pre-coated wells, with a 100 µL sample volume loaded per well in duplicate. A standard curve is generated in parallel with each run to convert absorbance readings into protein concentration values, supporting comparative studies across treatment groups, patient cohorts, or genetically modified model systems. Intended for research use only and not for diagnostic procedures. Reagent components should remain refrigerated between uses, with the lyophilized standard reconstituted, aliquoted, and stored frozen to limit repeated freeze-thaw cycles.