Human Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1) ELISA Kit

Quantitative measurement of the MHC class II DQ beta chain encoded by HLA-DQB1 in tissue homogenates and cell lysates is the purpose of this 96 assays immunoassay from Assay Genie. The HLA-DQB1 protein heterodimerizes with the HLA-DQA alpha chain to form the DQ antigen-presenting complex displayed on B lymphocytes, macrophages, and dendritic cells, where it presents exogenous peptide fragments to CD4-positive helper T cells and shapes adaptive immune responses.

This kit supports research into autoimmune disease genetics, particularly investigations of DQB1 allele associations with celiac disease and type 1 diabetes mellitus, where DQB1*02 and DQB1*03:02 are well-characterized susceptibility variants. It is also applied in transplantation immunology workflows profiling donor and recipient MHC compatibility, and in immunogenicity studies for vaccine and biologics development.

The 96 assays plate provides capacity for full standard curves alongside experimental samples run in duplicate or triplicate. Reconstituted standards and detection antibody should be stored refrigerated between uses, and all components should reach room temperature before the assay is started. Researchers using this kit for ELISA workflows should follow the package insert carefully for accurate sample preparation and quantitative readout. The reagents are intended for research use only and are not validated for diagnostic procedures.