Human Major Histocompatibility Complex Class I A (MHCA) ELISA Kit

The HLA-A gene on chromosome 6p21.3 encodes the heavy chain of Major Histocompatibility Complex (MHC) Class I molecules, presenting endogenous peptide fragments on the cell surface for surveillance by CD8-positive cytotoxic T lymphocytes. This Assay Genie quantitative immunoassay enables researchers to measure HLA-A protein concentrations in tissue homogenates and related biological matrices, supporting studies in transplantation immunology, autoimmune disease mechanisms, viral immune evasion, and tumor antigen presentation.

The plate-based format provides reagents sufficient for 96 individual determinations, with a chromogenic enzymatic reaction read as absorbance on a standard microplate spectrophotometer. Matched antibody pairs specific to human HLA-A capture the target from prepared samples, and bound analyte is quantified against a standard curve generated from the kit's reference preparations. Pre-coated plates, standards, detection antibody, and substrate components are organized for straightforward workflow integration in immunology and cell biology laboratories.

Investigators working on graft-versus-host responses, vaccine development, or MHC class I assembly pathways can incorporate this assay into downstream applications such as western blot confirmation, flow cytometry validation, or immunohistochemistry of matched tissue sections.