Human MAGED4 ELISA Kit (HUFI03522)

From Assay Genie, this ELISA kit enables quantitative measurement of MAGED4 (MAGE Family Member D4) protein in human research samples. MAGED4 is a melanoma-associated antigen family member involved in tumor biology, with documented roles in glioma cell proliferation, breast cancer prognosis, and apoptosis regulation. The protein is normally expressed in testis tissue but reactivated in certain malignancies, making it a target of interest for cancer biomarker discovery and tumor immunology studies.

The kit is supplied as a complete set of reagents in a 96 Assays packaging format, including a pre-coated microplate, lyophilized standards, detection antibodies, and substrate solution. Researchers should store components refrigerated upon arrival and protect from light to maintain stability. Reconstituted reagents are commonly aliquoted and stored frozen to limit freeze-thaw cycles during extended studies.

For research use only, the workflow accommodates small microliter sample volumes added to antibody-coated wells, followed by incubation with a biotinylated detection reagent and HRP conjugate for absorbance-based readout. The assay supports investigations of MAGE-family regulation, epigenetic reactivation in tumors, and serum or plasma-based biomarker discovery.