Human MAGEA11 ELISA Kit (HUFI03536)

The MAGEA11 protein (MAGE Family Member A11) is a cancer/testis antigen normally restricted to testicular germ cells in healthy adults, but frequently re-expressed in prostate carcinoma, melanoma, breast tumors, and non-small cell lung cancer. Quantifying circulating MAGEA11 levels with this Assay Genie kit supports investigations into tumor-associated antigen biology, androgen receptor signaling, and immune evasion mechanisms in translational oncology research.

This Assay Genie ELISA reagent set is supplied as a complete microplate-format package, including pre-coated capture wells, biotinylated detection antibody, dilution buffer, wash concentrate, chromogenic substrate, and stop solution. The standard antibody-based workflow uses 100 µL of sample per well, with diluted standards and specimens added to coated wells, followed by sequential incubation steps with detection reagents before chromogenic readout. Unopened components should be stored refrigerated at 4°C, protected from light, and used within the stated shelf life. For in vitro research use only.

Researchers commonly apply this kit to monitor MAGEA11 levels in cancer patient cohorts, stratify tumor samples, and explore correlations between antigen expression and clinical outcomes. The workflow is straightforward, and absorbance measurements provide quantitative readouts appropriate for downstream statistical analysis.