Human MADD (MAP kinase-activating death domain protein) ELISA KIT (HUFI05073)

The Assay Genie MADD ELISA kit enables quantitative measurement of MAP kinase-activating death domain protein in human samples using a precoated microplate. MADD, also designated DENN1A, is a critical adaptor in TNF receptor-1 and TRAIL receptor signaling, coupling death receptor activation to downstream MAPK cascades and caspase-dependent apoptosis; quantifying MADD supports research into neurodegeneration, ischemic injury, and tumor cell survival pathways. The kit format includes a strip plate, biotinylated detection antibody, standard diluent, wash buffer concentrate, and streptavidin-HRP conjugate. Standard curve range and analytical sensitivity are reported in the lot-specific certificate of analysis supplied with the kit, allowing laboratories to verify performance against validated controls before running experimental samples. The assay is intended for in vitro research use only and is not validated for diagnostic procedures. Reagents should be stored refrigerated between uses, with the standard kept frozen for long-term stability, and repeated freeze-thaw cycles of prepared working solutions avoided. Researchers studying apoptotic signaling, glioblastoma biology, and inflammatory cytokine networks frequently pair MADD quantification with parallel measurements of TNF-alpha, caspase-8, and ERK phosphorylation to map receptor-proximal death signaling events.