Human MAD2L1BP ELISA Kit (HUFI03545)

The MAD2L1BP gene (Gene ID 9587) maps to chromosome 6p21 and encodes a 31 kDa protein (UniProt accession Q15013) that is the mammalian ortholog of yeast Mad3 and cooperates with the TRIP13 AAA-ATPase to recycle closed MAD2 conformers back to the open state at attached kinetochores.

Assay Genie supplies this quantitative immunoassay in a microplate format compatible with standard automated plate washers. Researchers have used analogous MAD2L1BP assays to characterize checkpoint silencing dynamics in tumor cell lines, primary tumor lysates, and patient-derived xenograft samples. The assay tolerates blood-derived serum fractions, plasma preparations, cell culture supernatant, and tissue homogenate matrices, and shows no significant cross-reactivity with homologous spindle checkpoint proteins MAD2L2 or MAD1L1. Bring all reagents to room temperature before use, refrigerate unopened components, and aliquot reconstituted standards to limit freeze-thaw cycles before the printed expiration date.