Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit

Assay Genie's Human MIP-3α (CCL20) Sandwich ELISA Kit is engineered for quantitative measurement of the CCL20 chemokine in human serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants. The assay detects MIP-3α across a detection range of 15.6 to 1000 pg/mL, with a sensitivity of less than 5.6 pg/mL, supporting accurate profiling of low-abundance inflammatory signaling. Each microplate well accommodates a 100 µL sample volume, and the colorimetric readout is captured on a standard plate reader after stopping the enzymatic reaction. The kit includes a pre-coated 96-well plate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, and wash buffer sufficient for the full microplate format.

Reconstitute reagents as indicated on the certificate of analysis, refrigerate unused strips, and avoid repeated freeze-thaw cycles of the lyophilized standard. Researchers investigating psoriasis, rheumatoid arthritis, inflammatory bowel disease, mucosal immunity, or CCR6-mediated lymphocyte trafficking can apply this human-specific capture antibody system to map chemokine gradients in vitro within research-use-only workflows.