Human Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

A chemokine-targeted ELISA from Assay Genie that quantifies human Macrophage Inflammatory Protein 1 Beta (MIP-1β), also annotated as CCL4 with UniProt accession P13236. The 96 assays format supplies a pre-coated microplate, biotinylated detection antibody, lyophilized recombinant standards, and assay buffer sufficient to complete the workflow. Each well receives 100 µL sample or standard during the standard curve run, with timed incubations at 37 °C driving the capture-detection reaction to equilibrium.

Quantitative performance verified for this specific reagent includes sensitivity 5.5 pg/mL, detection range 15.625 to 1000 pg/mL, intra-assay coefficient of variation under 10%, and inter-assay coefficient of variation under 12%. Spike-and-recovery testing in human serum averaged 91% (range 83-100%), in EDTA plasma 96% (range 90-103%), and in heparin plasma 95% (range 89-102%), while serial dilution linearity of spiked samples remained between 80% and 104% across dilution factors of 1:2 through 1:16, supporting reliable interpolation from the standard curve.

Reactivity is restricted to human MIP-1β; under standard protocol conditions the antibody pair does not measurably cross-react with mouse, rat, or porcine homologs. Reconstituted standards should be aliquoted and held at -20 °C to limit freeze-thaw degradation, with the unopened kit shipped refrigerated and carrying a 12-month shelf life from the date of manufacture. Researchers deploy this reagent for profiling chemokine release in cell culture supernatants, mapping CCR5-mediated HIV co-receptor biology, characterizing autoimmune disease and transplant-rejection cohorts, and quantifying tumor-microenvironment signaling in oncology studies.