Human LZTFL1 ELISA Kit (HUFI03549)

Manufactured by Assay Genie, this Human LZTFL1 ELISA Kit supports quantitative measurement of Leucine Zipper Transcription Factor-Like 1 in human serum and plasma samples, and is supplied as a complete pack containing pre-coated microplate wells, lyophilized reference standards, biotinylated detection antibody, streptavidin-HRP conjugate, and chromogenic substrate. LZTFL1 regulates protein trafficking to the primary cilium and has been implicated in Bardet-Biedl syndrome, hedgehog pathway modulation, and host-cell susceptibility to SARS-CoV-2 infection, making the kit a useful tool for ciliopathy, retinal degeneration, and pulmonary biology research.

The protocol calls for a 100 uL sample volume dispensed into each well alongside a serial dilution of the supplied reference standard, followed by incubation, wash cycles, enzyme conjugate addition, and a chromogenic readout measured on a standard microplate reader. For long-term storage, the kit should be refrigerated upon arrival; reconstituted reagents should be aliquoted into separate tubes to minimize freeze-thaw cycles and preserve binding activity through the stated shelf life.

For laboratories interested in pathway-level readouts, LZTFL1 measurements can be paired with downstream PCR, western blot, or immunohistochemistry validation of related ciliary and trafficking markers. This product is intended for research use only and is not cleared for diagnostic procedures.