Human LY96 ELISA Kit (HUFI03553)

The LY96 protein, also called MD-2 (Myeloid Differentiation protein-2), is a 160 amino acid accessory glycoprotein that pairs with TLR4 on the cell surface and is required for lipopolysaccharide (LPS) recognition in innate immune signaling. This quantitative immunoassay from Assay Genie supports researchers investigating TLR4 pathway activation, endotoxin responses, and inflammation-associated biomarkers in clinical and translational studies.

Each kit ships with a pre-coated antibody plate, biotinylated detection antibody, recombinant standards, streptavidin-HRP conjugate, wash buffer, and substrate solution, all packaged together for the full plate run. The assay is intended for in vitro research use only. For optimal stability, store unopened components refrigerated and protect the substrate from light; reconstituted standards should be aliquoted into single-use vials to minimize freeze-thaw cycles, while the working conjugate is best kept away from prolonged ambient temperature exposure.

Common research applications include quantification of circulating MD-2 in studies of sepsis, septic shock, inflammatory bowel disease, atherosclerosis, and autoimmune conditions, alongside in vitro cell culture models examining TLR4 co-receptor regulation in macrophages and monocytes. The kit complements flow cytometry and western blot workflows when comparing patient cohorts, monitoring treatment response, or characterizing innate immune pathway activation.