Human LTF/Lactoferrin PharmaGenie ELISA Kit (SBRS0849)

Quantitative measurement of human lactoferrin in research-derived samples is the core purpose of this Assay Genie ELISA Kit. Lactoferrin is an ~80 kDa iron-binding glycoprotein of the transferrin family, secreted by glandular epithelial cells and released from the secondary granules of neutrophils during degranulation. The protein plays a frontline role in innate mucosal immunity, sequestering free iron to restrict microbial proliferation and modulating cytokine release, leukocyte chemotaxis, and barrier integrity. Common research applications include inflammation studies in sepsis, inflammatory bowel disease, cystic fibrosis, and acute respiratory infection, as well as investigations of mucosal defense, periodontal disease, and the contribution of neutrophil-derived mediators to chronic inflammation. The workflow accommodates clarified cell culture supernatant harvested from stimulated primary cells or relevant cell lines, plasma obtained from EDTA- or heparin-anticoagulated whole blood, and serum separated from clotted whole blood. The kit is supplied in a 96 assays configuration with a precoated microplate, recombinant human lactoferrin reference standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and substrate solution. Sample preparation typically involves clarifying plasma or serum at 1000 x g for 10-15 minutes prior to loading onto the plate. Researchers often pair lactoferrin readouts with other neutrophil and acute-phase markers such as calprotectin, MPO, or CRP to distinguish neutrophil-driven inflammation from hepatic responses. For research use only; not for diagnostic use.