Human LRP2BP ELISA Kit (HUFI03589)

Quantify LRP2BP (LDL receptor related protein 2 binding protein, also called Megalin-binding protein) in human-derived research samples with this ELISA kit from Assay Genie. LRP2BP functions as an intracellular partner of megalin (LRP2), the endocytic receptor expressed on renal proximal tubule epithelial cells that mediates reabsorption of filtered ligands, so measuring this protein is relevant to nephrology, endocytosis, and renal proximal tubule biology.

This 96 Assays kit includes a pre-coated microplate, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, substrate solution, and a recombinant human LRP2BP standard for generating a calibration curve. The protocol follows a two-antibody capture ELISA workflow with colorimetric read-out at the endpoint, suitable for research laboratories running standard plate readers. Reconstituted standards should be aliquoted to limit freeze-thaw cycles, and the conjugate should be protected from light; detailed reagent handling instructions accompany the product insert.

Applications include studies of proximal tubule function, proteinuria-related mechanisms, receptor trafficking in polarized epithelia, and characterization of cell lines expressing megalin and its cofactors. The kit is supplied as a single-use package sufficient for one full microplate of unknowns, standards, and controls.