Human LRP-6/Low-density lipoprotein receptor-related protein 6 ELISA Kit

A Sandwich ELISA, Double Antibody kit from Assay Genie is designed for the accurate detection of LRP-6 (Low Density Lipoprotein Receptor-Related Protein 6) levels in human samples. The assay is highly sensitive and specific, suitable for measuring LRP-6 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. LRP-6 is a key protein involved in the Wnt signaling pathway, playing a crucial role in cell proliferation, differentiation, and development, and its dysregulation has been implicated in cancer, osteoporosis, and Alzheimer's disease.

The assay operates in a range of 0.313-20 ng/mL with a sensitivity of 0.188 ng/mL, and detection is carried out colorimetrically by measuring absorbance at 450 nm after TMB substrate development. Linearity has been validated in serum (93-105% across 1:2 to 1:8 dilutions), EDTA plasma (82-99%), and heparin plasma (82-99%), with recovery averaging 92% in serum, 91% in EDTA plasma, and 96% in heparin plasma. The kit should be stored at 2-8°C for 12 months, and the standard double-antibody sandwich protocol uses a biotin-labeled detection antibody followed by HRP-Streptavidin binding.

Synonyms recognised for the target include LRP6, Low Density Lipoprotein Receptor Related Protein, ADCAD2, and low density lipoprotein receptor-related protein 6, with the protein referenced under UniProt O75581. The kit is intended for research use in cell biology applications relating to Wnt signaling and associated disease pathogenesis.