Human LPXN ELISA Kit (HUFI03598)

This assay from Assay Genie quantitatively measures human leupaxin (LPXN), a cytoskeletal focal adhesion protein of the paxillin family. Encoded on chromosome 11q12.1, leupaxin contains four LD motifs and four LIM domains that mediate protein-protein interactions at integrin-based adhesion sites, influencing cell migration, spreading, and osteoclast differentiation.

The plate-based format accommodates 96 reactions per kit, with pre-coated wells, calibration standards, and a labelled detection antibody supplied for colorimetric readout. Researchers investigating prostate and breast carcinoma, bone resorption disorders, or leukocyte adhesion can apply this kit to clarified liquid biopsy specimens, with each microplate supporting parallel standard curves and unknown samples in a single run.

Kit components are shipped refrigerated and should be kept at the temperature indicated on the vial labels until first use. Reconstituted standards and the concentrated detection reagent should be aliquoted before freezing to limit repeated freeze-thaw cycles, and the working substrate solution should be protected from light during color development.