Human LPIN2 ELISA Kit (HUFI03595)

The Human LPIN2 ELISA Kit from Assay Genie enables quantitative measurement of lipin-2, a magnesium-dependent phosphatidic acid phosphatase that catalyzes the penultimate step in triglyceride and phospholipid biosynthesis. Researchers studying glycerolipid metabolism, hepatic lipid handling, or Majeed syndrome—an autoinflammatory disorder driven by LPIN2 loss-of-function mutations—can use this microplate assay to quantify circulating lipin-2 in clinical research samples. The assay employs a pair of monoclonal antibodies recognizing distinct epitopes of human lipin-2, with no significant cross-reactivity observed against the closely related paralog LPIN1, supporting human-specific detection. Each kit includes a precoated microplate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, and wash buffer sufficient for 96 determinations. The procedure uses a sample volume of 100 µL per well and is compatible with standard laboratory plate readers. Reagents are shipped ready to use and should be stored at refrigerated conditions until first opening.