Human LOXL2/Lysyl Oxidase Homolog 2 ELISA Kit

This ELISA kit from Assay Genie enables quantitative measurement of human LOXL2 (Lysyl Oxidase Homolog 2) using a sandwich ELISA, double antibody detection format. It is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a sensitivity of 22.5 pg/mL and a working range of 37.5–2400 pg/mL.

The kit supports research into LOXL2, a key enzyme involved in extracellular matrix remodeling with critical roles in tissue repair, fibrosis, and cancer progression. Its dysregulation has been linked to a range of diseases, making it a relevant biomarker for disease diagnosis and therapeutic development. The product is categorized for research in epigenetics and nuclear signaling.

Validated performance includes recovery of 93–101% (average 98%) in serum, 92–102% (average 95%) in EDTA plasma, and 86–99% (average 93%) in heparin plasma. The procedure involves equilibrating reagents, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB color development in the dark for 10–20 minutes, and absorbance measurement at 450 nm after addition of stop solution. Kit components include a pre-coated microplate, lyophilized standard, biotin-labeled antibody, HRP-Streptavidin conjugate (SABC), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, wash buffer, plate sealer, and a technical manual. The kit should be stored at 2–8°C and remains stable for 12 months.