Human Low-density lipoprotein receptor-related protein 4/LRP4 ELISA Kit

A colorimetric Sandwich ELISA from Assay Genie designed for the quantitative measurement of human Low-Density Lipoprotein Receptor-Related Protein 4 (LRP4) in biological samples. The double-antibody sandwich design offers a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL, supporting reliable detection of LRP4 levels for research purposes.

Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is intended for use with human specimens and is stored at 2-8°C for up to 12 months. LRP4 is a key protein involved in lipid metabolism and cell signaling, with dysregulation linked to atherosclerosis, Alzheimer's disease, and certain types of cancer, supporting its value as a potential biomarker for disease research and therapeutic development.

Reported linearity at 1:2, 1:4, and 1:8 dilutions falls within 80-104% for serum, 82-104% for EDTA plasma, and 82-101% for heparin plasma (n=5 each). Recovery ranges from 85-105% (average 95%) for serum, 89-103% (average 95%) for EDTA plasma, and 88-103% (average 93%) for heparin plasma. The protocol uses a pre-coated microplate with biotin-labeled detection antibody, HRP-streptavidin binding, and TMB substrate development, with absorbance measured at 450 nm.