Human Lipocalin-2 PharmaGenie ELISA Kit (SBRS0837)

Quantify human Lipocalin-2 (also called NGAL, Neutrophil Gelatinase-Associated Lipocalin), a 25 kDa acute-phase glycoprotein central to innate immunity and iron homeostasis, with this colorimetric microplate assay from Assay Genie. Because Lipocalin-2 surges in circulation and urine during acute kidney injury and tracks closely with sepsis severity, it is a staple readout in nephrology research, critical-care biomarker studies, and translational investigations of renal protection. The protein is also studied in oncology, where elevated expression marks aggressive tumors, as well as in metabolic disease and chronic inflammation, so the kit fits cell-based work, animal-model studies, and human cohort screening alike. The 96-assay plate configuration supports multiple sample replicates and a full standard curve in a single run, while the colorimetric readout is compatible with standard microplate readers commonly found in research laboratories. Sample compatibility covers cell culture supernatants, EDTA or heparin plasma, and serum drawn from research subjects, accommodating both in vitro experiments and clinical research protocols. Each kit provides pre-coated assay wells, concentrated standards, biotinylated detection reagent, enzyme conjugate, wash buffer, substrate, and stop solution sufficient for one full plate. For best results, refrigerate kit components between uses, allow reagents to equilibrate to room temperature before running the assay, protect the substrate from light, and avoid repeated freeze-thaw cycles of reconstituted standards. Assay Genie supplies a detailed protocol with recommended dilutions and standard curve ranges to support reproducible quantification of this clinically relevant biomarker.