Human LIPA ELISA Kit (HUFI03611)

Lysosomal acid lipase (LIPA) is the hydrolytic enzyme responsible for breaking down cholesteryl esters and triglycerides inside the lysosome. Deficiency in LIPA activity underlies two clinically distinct lipid-storage disorders, Wolman disease in infants and Cholesteryl Ester Storage Disease in older patients, both characterized by accumulation of lipid-laden macrophages in liver, spleen, and intestinal wall. The enzyme therefore sits at a critical intersection between intracellular lipid handling and systemic cholesterol homeostasis, and quantitative measurement of LIPA protein is widely used to model disease severity and treatment response in research settings.

This Assay Genie ELISA Kit is a colorimetric plate-based immunoassay designed to quantify LIPA protein in human research specimens, supporting studies into lysosomal storage disease biology, atherosclerosis-related lipid metabolism, and hepatic steatosis. The kit is supplied in a microplate format intended for research use only and provides the standard curve, detection antibody, wash buffer, and other assay components needed to run the protocol end-to-end. The workflow follows a standard sandwich-style capture-and-detection approach, with absorbance read on a standard plate reader, making it compatible with most clinical and academic research laboratories.

Researchers working on LAL deficiency, Niemann-Pick type C comparison studies, or hepatic lipid handling can use LIPA measurements alongside cholesterol ester profiling, hepatic enzyme panels, and lipidomics readouts to investigate disease mechanisms, genotype-phenotype correlations, and the impact of investigational enzyme replacement strategies in cellular and animal models.