Human LIMD2 ELISA Kit (HUFI03621)

This research-use-only immunoassay from Assay Genie enables quantification of LIM Domain Containing 2 (LIMD2) protein in human samples, supporting investigators studying focal adhesion biology, cytoskeletal remodeling, and tumor metastasis pathways. LIMD2 has been characterized in peer-reviewed oncology research as a focal adhesion-associated protein linked to cell motility and epithelial-to-mesenchymal transition, with elevated levels reported in metastatic lung adenocarcinoma and head and neck squamous cell carcinoma models. The protein's role in adhesion complex dynamics makes it a relevant target for cancer research laboratories, cell biology programs, and translational biomarker studies. Each kit is supplied with pre-coated microplate strips, biotinylated detection antibody, recombinant protein standards, streptavidin-HRP conjugate, and a complete protocol insert sufficient for 96 determinations. Reconstituted standards and diluted samples should be aliquoted to limit freeze-thaw cycles, and unused strip wells returned to the foil pouch with desiccant and refrigerated at 4 °C to preserve antibody binding activity. The workflow is compatible with automated plate washers and standard microplate readers, with all reagents validated for in vitro research use only, excluding diagnostic or clinical decision-making. Suitable applications include pathway-focused cancer research, validation of LIMD2 expression changes following siRNA knockdown or CRISPR editing in cell culture systems, and screening of compound libraries for modulators of adhesion signaling.