Human LIG4 ELISA Kit (HUFI03617)

DNA Ligase 4 (LIG4) is the catalytic core of the non-homologous end joining pathway, sealing broken DNA ends during double-strand break repair and V(D)J recombination in developing lymphocytes. The Assay Genie Human LIG4 ELISA Kit enables quantitative measurement of this enzyme in human research samples, supporting studies of genome stability, radiation response, and inherited immunodeficiencies such as LIG4 syndrome.

Each kit supplies reagents sufficient for 96 individual reactions, including a precoated plate, calibrated reference standards, detection antibody, wash buffer, and substrate solution. The plate-based protocol integrates into standard laboratory workflows and is read on a conventional microplate reader after substrate development. Researchers running cohort studies or cell-line panels benefit from the batched format, which supports multiple sample sets in a single run.

Unopened kits are shipped cold and should be stored refrigerated to preserve reagent activity across the stated shelf life. The assay is calibrated for human LIG4 specifically and is labelled for research use only, without validation for clinical diagnostic applications.