Human LIG1 (DNA ligase 1) ELISA KIT (HUFI05093)

DNA ligase 1 is the enzyme responsible for sealing nicks in duplex DNA during replication and long-patch base excision repair, joining Okazaki fragments on the lagging strand. This Assay Genie kit provides quantitative measurement of LIG1 protein in circulating blood specimens using a microplate-based protocol, supporting studies in DNA repair capacity, genomic stability, and oncology biomarker research where altered LIG1 expression has been linked to tumor progression and chemoresistance.

The assay procedure accommodates a 100 uL sample volume per well, with results read on a standard plate reader. Reconstituted reagents and prepared standards remain stable through multiple freeze-thaw cycles when aliquoted, and unopened kit components should be stored at 4 degrees C protected from light to preserve antibody activity across the shelf life of the product.