Human LIF DIY ELISA Kit

The Human LIF DIY ELISA Kit from Assay Genie supplies all essential reagents for quantifying Leukemia Inhibitory Factor in cell culture supernatants and other experimental samples. The DIY (Do-It-Yourself) format includes capture antibody, biotinylated detection antibody, recombinant human LIF standard, and streptavidin-HRP, allowing researchers to coat their own microplate wells and configure layouts to suit their experimental workflow. With reagent volumes sufficient for up to 10 plates, this kit offers flexibility for high-throughput screening or longitudinal studies tracking LIF expression.

LIF (Leukemia Inhibitory Factor) is a pleiotropic cytokine belonging to the IL-6 family, signaling through the LIF receptor (LIFR) and the co-receptor gp130 to activate JAK/STAT3, MAPK, and PI3K pathways. It plays critical roles in mouse embryonic stem cell pluripotency maintenance, inflammatory responses, bone remodeling, neural development, and reproductive biology. Measurement of human LIF levels is widely used in regenerative medicine research, oncology investigations, neurobiology, and inflammation models.

Reagents should be stored refrigerated at 4 degrees C upon receipt; the reconstituted standard should be aliquoted and stored frozen to minimize freeze-thaw cycles. This kit is intended for research use only and in vitro applications. The protocol yields an absorbance-based readout on a standard microplate reader equipped with an appropriate filter.