Human LH PharmaGenie ELISA Kit (SBRS0830)

The Human LH PharmaGenie ELISA Kit from Assay Genie is a sandwich-format immunoassay for quantitative measurement of luteinizing hormone in human biological samples. Using a pre-coated 96-well microplate, the assay delivers colorimetric readout at 450 nm and accommodates serum, plasma, and cell culture supernatant specimens. Researchers in reproductive endocrinology, fertility studies, pituitary function, and gonadotropin regulation use this assay for reproducible LH quantification.

Key specifications include a detection range of 0.156 to 10 mIU/mL, sensitivity below 0.1 mIU/mL, and a recommended sample volume of 100 uL per well. Unopened kits should be stored at 2 to 8 degrees C; reconstituted reagents and standards require storage at -20 degrees C. The standard curve uses a seven-point serial dilution with intra-assay and inter-assay precision reported as percentage CV.

Each kit contains a pre-coated microplate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and assay diluent. The protocol is for research use only and is not validated for clinical diagnostic applications.