Human LGI3 ELISA Kit (HUFI03626)

Quantitative measurement of human Leucine-Rich Glioma-Inactivated Protein 3 is enabled by the Assay Genie Human LGI3 ELISA Kit, a plate-based immunoassay relevant to neuroscience and oncology research. LGI3 is a secreted member of the LGI protein family that has been investigated in neuronal development, axon guidance, and tumor-related signaling pathways, giving the assay broad relevance for biomarker discovery, mechanistic studies of brain-related disorders, and exploratory cancer research.

Configured to accept serum-derived samples, the kit also accommodates conditioned media from cell culture experiments, tissue lysates, and other validated research matrices, although users should refer to the supplied protocol for guidance on preparation, dilution, and matrix-specific considerations. Each microplate supplies reagents for 96 individual determinations, and the readout relies on a standard spectrophotometric workflow compatible with routine microplate readers in biomedical laboratories. Researchers can generate standard curves from serial dilutions of the calibrator provided.

Assay Genie supplies pre-coated plates, assay diluent, detection antibody, standard calibrators, substrate, wash buffer, and stop solution in quantities matched to the 96-assay run. Unopened components should be refrigerated upon arrival, while working aliquots may be kept at colder temperatures for repeated use. Researchers are encouraged to refer to the lot-specific certificate of analysis and the package insert for storage conditions, dilution recommendations, and any assay-specific notes that influence curve-fitting and final concentration calculations.

Typical applications include profiling LGI3 expression in neuronal cell models, screening clinical research specimens for LGI3 levels, and tracking changes in LGI3 abundance after experimental manipulation. Because LGI3 is secreted, the assay is particularly useful for monitoring release into cell culture supernatants and for in vitro signaling studies that examine its role in neuron-glia communication and related pathways. The kit is intended for research use only and is not cleared for diagnostic procedures.