Human Leukotriene B4 receptor 1/LTB4R ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit uses a double-antibody method for the precise measurement of Human Leukotriene B4 receptor 1 (LTB4R) levels. The assay is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a reported sensitivity of 0.469 ng/mL and a measuring range of 0.781–50 ng/mL. Reagents are stored at 2–8°C for up to 12 months.

The procedure involves a series of incubations on a pre-coated plate, beginning with a 90-minute antigen-binding step at 37°C, followed by incubation with a biotin-labelled detection antibody and HRP-Streptavidin conjugate, and detection using TMB substrate with absorbance read at 450 nm. Sample preparation guidance is provided for serum, plasma, tissue homogenates, cell culture supernatants and cell lysates.

LTB4R is a key mediator of the inflammatory response, contributing to leukocyte recruitment and activation. Its dysregulation has been implicated in conditions such as asthma, arthritis and inflammatory bowel disease, making accurate quantification of LTB4R valuable for research into these inflammatory conditions and potential therapeutic targets.