Human Leukemia inhibitory factor (LIF) ELISA Kit

This sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of human Leukemia Inhibitory Factor (LIF) in research samples. LIF is a pleiotropic cytokine involved in stem cell maintenance, embryo implantation, and immune response regulation, with dysregulation linked to conditions such as leukemia and inflammatory disorders, making it a valuable biomarker for disease research.

The kit provides a detection range of 31.2-2000pg/mL with a sensitivity of 12pg/mL. Precision is reported as 3.9% intra-assay CV and 6.5% inter-assay CV. Linearity has been validated in serum, EDTA plasma, and heparin plasma across dilutions from 1:2 to 1:16. Recovery averages 84% in serum (range 80-90%) and 86% in plasma (range 80-92%).

The assay accepts serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids, and shows specificity for natural and recombinant human LIF. Recognised synonyms include Differentiation-stimulating factor, Melanoma-derived LPL inhibitor, D factor, MLPLI, and HILDA. The product is intended for research use only, with applications in cancer research and the study of secreted signalling molecules.